FIGURE 2.

Design and characters of the synthetic malonyl-CoA sensors. (A) Design of the synthetic promoters. The positions of fapO sequence were as follows: 7 bp downstream the TATA box of GAL1 core promoter, immediately upstream the TATA box and 61 bp downstream the TATA box of GAL1 core promoter. (B) Comparing of the fluorescence intensity of the sensors between with FapR and without FapR. The synthetic promoters were cloned in multiple-copy plasmid. Fluorescence intensity (a.u.) means total fluorescence normalized by cell density (OD600). (C) The fluorescence intensity fold change of the sensors with gradient concentration of cerulenin addition relative to zero cerulenin addition. HFapR: fapR was expressed in a multicopy plasmid; LFapR: fapR was integrated into genome.