Figure 1.

N-methyl-D-aspartate (NMDA) receptor-dependent long-term potentiation (LTP) was impaired in exercise-induced fatigue (EF) mice. (A) Image of a live coronal corticostriatal slice taken with a 4× objective showing the placement of stimulating and recording electrodes. (B1) LTP induced by high-frequency stimulation (HFS) in Mg²⁺-free artificial cerebrospinal fluid (ACSF) was impaired in EF mice. (B2) Sample traces (average of 20 consecutive sweeps) taken at baseline (1) and the last 10 min recording (2). (B3) The average field excitatory postsynaptic potential (fEPSP) amplitudes of the last 10 min recording (35–45 min in A1) were used for the plot. (C) Corticostriatal LTP in control mice was blocked by NMDA receptor antagonist APV (50 μM). All data are showed as mean ± SEM. Student’s t-test, **p < 0.01.