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. 2018 Jan 25;9:40. doi: 10.3389/fphys.2018.00040

Figure 4.

Figure 4

Myocardial CKIP-1 overexpression protects from simulated microgravity induced-cardiac atrophy. (A) H&E-stained sections of hearts from WT and CKIP-1 TG mice after 28 days of hindlimb unloading. Sections of hearts are stained with Masson trichrome (MTT) to detect fibrosis (blue). Wheat germ agglutinin (WGA) staining is used to demarcate cell boundaries. Scale bars: 50 μm. (B) The cardiomyocyte crosssectional area was measured from 8-μm-thick heart sections that had been stained with WGA by using ImageJ software (NIH). Only myocytes that were round were included in the analysis. The studies and analysis were performed blinded as to experimental. Data represent the means ± SEM (n = 6), *P < 0.05, **P < 0.01. (C–E) The mRNA levels of Col1a1, Col3a1, and BNP were analyzed by Q-PCR from WT and CKIP-1 TG mice after 28 days of hindlimb unloading. The relative abundance of transcripts were quantified and normalized to GAPDH. Data represent the means ± SEM (n = 6), *P < 0.05.