Figure 8.

In vivo perfusion study showing ${\text{HCO}}_3^{-}$ transport by endometrial epithelium of rat uterus. (A) Diagram for in vivo perfusion of rat uterus. For perfusate delivery, a perfusion tube was cannulated at the distal end of the right uterus horn. For sample collection, a collecting tube was cannulated at the proximal end of the right uterus horn. (B) Changes in [${\text{HCO}}_3^{-}$] of the luminal perfusate in rat uteri when perfused with Ringers' solution containing 5% CO₂/50 mM ${\text{HCO}}_3^{-}$. (C) Rate of ${\text{HCO}}_3^{-}$ reabsorption (J_HCO3^Abs) by endometrial epithelium at estrus and diestrus. (D) Changes in [${\text{HCO}}_3^{-}$] of the luminal perfusate in rat uteri when perfused with nominally ${\text{HCO}}_3^{-}$-free HEPES solution. (E) Rate of ${\text{HCO}}_3^{-}$ secretion (J_HCO3^Scr) by endometrial epithelium at estrus and diestrus. In: Influent fluid; Out: Effluent fluid. Stars in B,D indicate significance between estrus and diestrus. Student's t-test was performed for statistical comparison between estrus and diestrus. ^*p < 0.05; ^**p < 0.01.