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. 2018 Jan 29;8:1754. doi: 10.1038/s41598-018-19976-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relationship between disease severity and TNF-α release in Bacteria and PWM assay. TNF-α release from whole blood was correlated with quartile groups for disease severity as measured by SAPS II (A,C) and APACHE II (B,D) disease severity classification systems. Panels A and B show hereby stimulations with bacterial antigen mixtures, Panels C and D with Pokeweed mitogen assay. Statistically significant differences (One-way ANOVA on RANKS followed by Dunn’s test) are indicated *p < 0.05.