Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 29;8:1793. doi: 10.1038/s41598-018-20067-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Deletion of the nine final Mfa3 residues does not affect the polymerization or downstream proteins. Expression of (A) Mfa3 or (B) Mfa1 or (C) polymerization of Mfa1. Whole cell lysates were solubilized in SDS buffer, heated to 100 °C for 5 min (A and B) or 60 °C for 10 min (C). The samples were separated on SDS-PAGE, blotted to a PVDF membrane and probed with a polyclonal Mfa3 antibody (A) or an Mfa1 fimbriae antibody (B and C). Lanes: 1, JI-1 (positive control); 2, Δmfa3ΔfimA; 3, +mfa3; 4, +mfa3ΔC (A–C). Effect of mfa3ΔC mutation on incorporation of accessory proteins (D–F). (D) SDS-PAGE of pure Mfa1 fimbriae. (E) Immunoblot analysis of pure Mfa1 fimbriae using an anti-Mfa3 antibody. (F), Immunoblot analysis of pure Mfa1 fimbriae using an anti-Mfa4 antibody Lanes: 1, JI-1 (positive control); 2, Δmfa3ΔfimA; 3, +mfa3ΔC (D–F).