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. 2018 Jan 29;38(4):e00476-17. doi: 10.1128/MCB.00476-17

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FIG 2 — GFZF is associated with polytene chromosomes. (A) Polytene chromosomes from third-instar larvae were spread and stained with antibody against GFZF. (B) Comparison of M1BP and GFZF staining patterns at the end of chromosome 2R on separately stained samples. White lines guide an alignment of the patterns. (C and D) Polytene chromosomes from third-instar larvae expressing M1BP with N-terminal double FLAG and single HA tags under the control of the Hsp83 promoter were spread and stained with rabbit antibody against M1BP (C) and mouse monoclonal antibody against the FLAG epitope (D). (E) Polytene chromosomes from flies expressing 2×FLAG-HA-M1BP were spread and stained with mouse antibody against the FLAG epitope and rabbit antibody against GFZF. For ease of comparison, the images were split and aligned so that GFZF is to the left of the thin white line and FLAG-M1BP is to the right.