Table 2.
The efficiency and fidelity of creating substitutions with LFEAP mutagenesis.
| Gene | Gene ID | Size (bp) | Vector | Mutation | CFU/µg DNAa | Positive (%)b |
|---|---|---|---|---|---|---|
| yaaU | 944766 | 1,332 | pNGFP-BC | DE224AA | 7375 ± 18 | 100 ± 0 |
| GAST | 2520 | 306 | pNGFP-EU | SQQ27AAA | 7296 ± 16 | 96.7 ± 5.8 |
| yaaU | 944766 | 1,332 | pNGFP-BC | RKGRVKECEE202AAAAAAAAAA | 8178 ± 21 | 100 ± 0 |
| GAST | 2520 | 306 | pNGFP-EU | EQQGPASHHRRQ48AAAAAAAAAAAA | 7827 ± 15 | 100 ± 0 |
aReported results are the mean ± s.d. of three independent experiments.
bFor each independent experiment, ten of colonies were checked by DNA sequencing.