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. 2018 Jan 29;8:1768. doi: 10.1038/s41598-018-20031-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Different representations of syncytia size distribution. RAW264.7 cells were committed to osteoclastogenesis by RANKL application at t = 0. LPC was applied at t = 72 h and removed at t = 88 h (LPC wash). Number of nuclei per syncytia was analyzed at t = 89.5 h (i.e., 90 min after LPC removal) as described in methods. At the time of LPC removal cells were treated with different concentrations of MiTMAB or not treated with MiTMAB (“LPC Wash”) cells. In the negative control experiment (“LPC on”), LPC was not removed. (A) Bar heights represent numbers of syncytia within ranges of nuclei per syncytia denoted on X-axis. Different experimental conditions are shown in different color. (B) Empirical CDFs of number of nuclei per syncytia are shown in different color for different experimental conditions.