Skip to main content
. 2018 Jan 29;8:1808. doi: 10.1038/s41598-018-20052-6

Figure 6.

Figure 6

Brain and behavioral deficits persist in Asah1P361R/P361R;MCP-1−/− mice. Representative micrographs from 8–9-week-old and 11–12-week-old mice from all genotypes featuring cerebellum, thalamus, and cortex regions stained for GFAP and Iba-1. Original magnification of cerebellum samples is 10x. The scale bar represents 100 µm and the original magnifications of the thalamus and cortex samples were 20x  wherein the scale bar represents 50 µm (A–E). The percentage of GFAP and Iba-1 positive staining from the cerebellum, thalamus, and cortex regions. Quantitation was performed on micrographs from 8–9 and 11–12-week-old mice. n = 3–4 mice per genotype (F and G). Brain wet-to-dry weight ratios were performed on 8–9 and 11–12-week-old mice. n = 3–4 mice per genotype (H). Behavioral tests were performed on 8–9 and 11–12-week-old mice for grip strength (I–L) and Rotarod proficiency (M). n = 8–10 per genotype. ns (not significant), *p < 0.05 **p < 0.01, ***p < 0.001.