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. 2018 Jan 29;9:419. doi: 10.1038/s41467-018-02818-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Mechanisms of membrane bending during CME and clathrin ultrastructure in unroofed SK-MEL-2 cells. a Schematic representation of CME in which membrane bending proceeds with a fixed radius of curvature during the addition of clathrin subunits. b Representation of CME in which clathrin first assembles into a flat sheet that remodels into a vesicle. c Correlative dSTORM and platinum-replica TEM images of fluorescently labeled clathrin (magenta) demonstrate a range of heterogeneous topographies even at the earliest stages of CME; scale bar is 200 nm. d The size distribution observed amongst clathrin structures in WT SK-MEL-2 cells (black) or SK-MEL-2 cells exogenously expressing clathrin light chain for correlative microscopy (blue). The inset shows ratios of average fluorescence associated with round vs. domed structures and domed vs. flat structures (SD shown for N = 3 cell membranes)