(A) Cell growth was detected by MTT method at 0, 24, 48, 72, and 96 h point. (B) The expression of CD133 protein was assessed by Western blot in CD133− SGC7901 and CD133+ SGC7901 cells. (C) Representative images of gastric cancer cell (SGC7901) migration and quantized histogram of relative migrating SGC7901 cells. (D) MKN45 cell growth was detected by MTT method at 0, 24, 48, 72, and 96 h point. (E) The expression of CD133 protein was assessed by Western blot in CD133− MKN45 and CD133+ MKN45 cells. (F) Representative images of gastric cancer cell (MKN45) migration and quantized histogram of relative migrating MKN45 cells. All data were expressed as mean ± standard error (SE); *P<0.05, **P<0.01 vs CD133− SGC7901 cells. β-Actin was used for internal control in Western blot analysis.