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| Nanoparticle | NPs (PEG-PLGA) | Pemetrexed-NPs (PEG-PLGA-pemetrexed) | METase/Pemetrexed-NPs (PEG-PLGA-METase-pemetrexed) |
|---|---|---|---|
| W1 | PBS/Tween (8:2) Pemetrexed | PBS/Tween (8:2) pcDNA-ME Tase Pemetrexed | |
| O | DCM | DCM | DCM |
| PEG-PLGA polymer | PEG-PLGA polymer | PEG-PLGA polymer | |
| W2 | 3% PF68 (w/v) | 3% PF68 (w/v) | 3% PF68 (w/v) |
W1 means internal aqueous phase, O means oil phase, W2 means external aqueous phase, and DCM means dichloromethane.
