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. 2018 Jan 3;7(1):3. doi: 10.3390/cells7010003

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Visual quality control for plating of antigen. (A) The plate layout for an experiment is shown in which 47 antigens are to be tested on a plate, each in duplicate wells. The four selected reagent tracker dyes have been assigned to the antigens in a revolving order, as shown by the four background colors assigned to the data fields. Wells A1 and A2 are the negative controls, with cells in phenol red containing medium only, without antigen/RT dye added; (B) A back-lit photograph of the ELISPOT plate is shown after the RT-dye-labelled antigens have been plated. The finalist dye concentrations were used, 0.39%, 0.39%, 0.09% and 1.56% for dyes C, D, E, and L, respectively in phenol red free medium.