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. 2018 Jan 19;7(1):18. doi: 10.3390/antiox7010018

Figure 1.

Figure 1

GC4419 increases the oxidative flux of systems containing AscH− (A) GC4419 increases [Asc•−]ss in complete media containing AscH−. Ascorbate alone or GC/AscH− were added to complete RPMI media. Solutions were immediately examined by EPR and [Asc•−]ss was determined. 3-Carboxyl-PROXYL was used as a concentration standard [25]. Errors represent ± SEM, n = 3. * p < 0.05, ** p < 0.01 by unpaired t-test. (B) GC4419 increases oxygen consumption of complete media containing AscH−. Ascorbate was added to complete RPMI media (Control) and the rate of oxygen consumption was measured. GC4419 was then added to the solution and oxygen consumption was measured. Errors represent ± SEM, n = 3. ** p < 0.01 by unpaired t-test.