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Catalytic activity of pentaazamacrocyclic mimetic is required to enhance AscH− toxicity as seen by clonogenic assay. Media were replaced and cells were treated with 10 μM of GC4419 or GC4404 (SOD inactive) for 1 h prior to 1 h AscH− treatment. Errors represent ± SEM, n = 4–7 with at least two cloning dishes per treatment. **** p < 0.0001, N.S. = no significance vs. Control by two-way ANOVA. Normalized to Control, GC4419, or GC4404 groups.
