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. 2018 Jan 20;7(1):19. doi: 10.3390/antiox7010019

Figure 1.

Figure 1

The replicative life span of WI-38 cells is affected by the selenium level of the culture medium. At cumulative population doublings (CPD) 35.8, WI-38 cells were plated in three different conditions, namely, Se-deficient (Dpl, black dots), Se-control (Ctl, blue square), and Se-supplemented (Sup, red square) for two passages and until they reached the senescent stage. The gray ellipses illustrate the time and stage the cells were harvested for 2-Dimensional Differential in-Gel Electrophoresis (2D-DIGE) analyses.