Skip to main content
. 2018 Jan 20;7(1):19. doi: 10.3390/antiox7010019

Table 1.

List of proteins identified by tandem mass spectrometry (MS/MS) (either by quadrupole-time-of-flight (Q-TOF) or Orbitrap) from the coomassie blue-stained gel (presented in Figure 3).

Master Spot Number Targets of Senescence Targets of Selenium in Young Cells Targets of Selenium in Senescent Cells
Protein Name Gene Name MS Ident Score Nb Nb acc Average Ratio (S/Y) p Value Average Ratio (Sup/Dpl) p Value Average Ratio (Sup/Dpl) p Value
Mascot Peptides
Proteins Targets Common to Three Conditions
754 Vinculin VCL Orbitrap 452 15 P18206 2.40 0.01800 2.68 0.01800 3.06 0.00540
2057 Ferritin light chain FTL Orbitrap 422 21 P02792 1.91 0.00260 −1.78 0.00046 −3.05 0.00000
1870 Superoxide dismutase [Mn], mitochondrial SOD2 Orbitrap 165 5 P04179 1.58 0.00016 −1.64 0.00057 −2.42 0.00086
1870 Ubiquitin carboxyl-terminal hydrolase isozyme L1 UCHL1 Orbitrap 197 12 P09936 1.58 0.00016 −1.64 0.00057 −2.42 0.00086
1869 Ubiquitin carboxyl-terminal hydrolase isozyme L1 UCHL1 Q-TOF 251 9 P09936 1.54 0.00010 −1.51 0.00013 −2.34 0.00003
Proteins Targets Common to Two Conditions
600 Glycyl-tRNA synthetase GARS Q-TOF 88 4 P41250 1.90 0.00059 −2.09 0.00150
600 Mitochondrial inner membrane protein IMMT Q-TOF 72 2 Q16891 1.90 0.00059 −2.09 0.00150
579 Glycyl-tRNA synthetase GARS Orbitrap 1047 47 P41250 1.64 0.00300 −1.63 0.00130
579 Mitochondrial inner membrane protein IMMT Orbitrap 1000 45 Q16891 1.64 0.00300 −1.63 0.00130
579 Vinculin VCL Orbitrap 940 31 P18206 1.64 0.00300 −1.63 0.00130
1546 Prohibitin-2 PHB2 Q-TOF 191 6 Q99623 4.48 0.00078 2.69 0.00140
789 78 kDa glucose-regulated protein HSPA5 Orbitrap 660 22 P11021 2.67 0.00280 4.47 0.00073
1500 L-lactate dehydrogenase B chain LDHB Orbitrap 235 6 P07195 2.61 0.00029 2.33 0.00010
1500 Malate dehydrogenase, cytoplasmic MDH1 Orbitrap 380 15 P40925 −2.61 0.00029 2.33 0.00010
761 Heat shock cognate 71 kDa protein HSPA8 Orbitrap 557 20 P11142 2.60 0.00540 1.79 0.01200
761 Stress-70 protein, mitochondrial HSPA9 Orbitrap 573 20 P38646 2.60 0.00540 1.79 0.01200
753 Gelsolin GSN Orbitrap 252 10 P06396 2.40 0.01100 2.80 0.00230
1385 Actin, cytoplasmic 1 ACTB Orbitrap 184 6 P60709 2.33 0.00099 1.96 0.01500
1385 Translation initiation factor eIF-2B subunit beta EIF2B2 Orbitrap 302 9 P49770 2.33 0.00099 1.96 0.01500
1382 Biliverdin reductase A BLVRA Q-TOF 97 5 P53004 2.27 0.00130 2.14 0.00510
753 Heat shock protein 70 kDa HSPA1A Orbitrap 252 7 P08107 2.21 0.01100 2.80 0.00230
752 Heat shock protein 70 kDa HSPA1A Orbitrap 687 24 P08107 2.21 0.01500 2.50 0.00300
1503 Desmoplakin DSP Orbitrap 198 10 P15924 2.21 0.00190 1.90 0.00095
1503 L-lactate dehydrogenase B chain LDHB Orbitrap 225 5 P07195 2.21 0.00190 1.90 0.00095
1503 Malate dehydrogenase, mitochondrial MDH2 Orbitrap 172 6 P40926 2.21 0.00190 1.90 0.00095
1383 Elongation factor 2 EEF2 Orbitrap 260 11 P13639 2.15 0.00120 2.42 0.01200
1383 PDZ domain-containing protein GIPC1 GIPC1 Orbitrap 244 7 O14908 2.15 0.00120 2.42 0.01200
1383 Transaldolase TALDO1 Orbitrap 164 5 P37837 2.15 0.00120 2.42 0.01200
1837 Peroxiredoxin-6 PRDX6 Q-TOF 214 11 P30041 2.04 0.00020 −1.54 0.01300
553 Neutral alpha-glucosidase GANAB Orbitrap 416 15 Q14697 1.85 0.00007 −1.64 0.00140
553 Programmed cell death 6-interacting protein PDCD6IP Orbitrap 482 18 Q8WUM4 1.85 0.00007 −1.64 0.00140
553 Caldesmon CALD1 Q-TOF 365 20 Q05682 1.85 0.00007 −1.64 0.00140
553 Neutral alpha-glucosidase AB GANAB Q-TOF 422 20 Q14697 1.85 0.00007 −1.64 0.00140
1635 Annexin A1 ANXA1 Orbitrap 486 12 P04083 1.84 0.00096 −2.54 0.00460
1635 Guanine nucleotide-binding protein subunit beta-2-like 1 GNB2L1 Orbitrap 647 22 P63244 1.84 0.00096 −2.54 0.00460
1635 Syntenin-1 SDCBP Orbitrap 747 22 O00560 1.84 0.00096 −2.54 0.00460
1211 Actin, cytoplasmic 2 ACTG1 Q-TOF 63 4 P63261 1.83 0.02500 1.81 0.00014
1734 Prohibitin PHB Q-TOF 249 10 P35232 1.76 0.00053 −1.63 0.00160
1859 Ubiquitin carboxyl-terminal hydrolase isozyme L1 UCHL1 Q-TOF 119 5 P09936 1.69 0.03500 −2.18 0.00023
1336 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial HIBCH Orbitrap 181 5 Q6NVY1 1.62 0.00014 −1.69 0.01700
1336 Methionine adenosyltransferase 2 subunit beta MAT2B Orbitrap 186 4 Q9NZL9 1.62 0.00014 −1.69 0.01700
1336 Phosphoserine aminotransferase PSAT1 Orbitrap 261 11 Q9Y617 1.62 0.00014 −1.69 0.01700
1644 Annexin A1 ANXA1 Q-TOF 198 5 P04083 1.52 0.00520 −1.91 0.00600
1644 Guanine nucleotide-binding protein subunit beta-2-like 1 GNB2L1 Q-TOF 110 4 P63244 1.52 0.00520 −1.91 0.00600
829 Pyruvate kinase isozymes M1/M2 PKM2 Q-TOF 299 14 P14618 −1.54 0.00200 1.70 0.00280
1486 Actin, alpha skeletal muscle ACTA1 Q-TOF 259 5 P68133 −1.60 0.00190 1.61 0.00044
1486 Actin, cytoplasmic 2 ACTG1 Q-TOF 259 12 P63261 −1.60 0.00190 1.61 0.00044
822 Pyruvate kinase isozymes M1/M2 PKM2 Q-TOF 152 9 P14618 −1.78 0.00130 2.59 0.00069
1381 Serine-threonine kinase receptor-associated protein STRAP Orbitrap 667 17 Q9Y3F4 −1.87 0.00004 1.65 0.02300
1381 Vimentin VIM Orbitrap 269 13 P08670 −1.87 0.00004 1.65 0.02300
720 Heat shock protein 75 kDa, mitochondrial TRAP1 Q-TOF 143 6 Q12931 −1.89 0.00056 2.23 0.00120
729 Heat shock protein 71 kDa HSPA8 Orbitrap 702 24 P11142 −2.01 0.00046 2.54 0.00110
729 Prelamin-A/C LMNA Orbitrap 577 23 P02545 −2.01 0.00046 2.54 0.00110
729 Heat shock protein 75 kDa, mitochondrial TRAP1 Orbitrap 783 32 Q12931 −2.01 0.00046 2.54 0.00110
2202 ADP-ribosylation factor 1 ARF1 Orbitrap 298 9 P00441 −2.18 0.00093 2.74 0.00160
2202 Cofilin-1 CFL1 Orbitrap 193 5 P23528 −2.18 0.00093 2.74 0.00160
2202 Superoxide dismutase [Cu-Zn] SOD1 Orbitrap 399 13 P00441 −2.18 0.00093 2.74 0.00160
Proteins Targets of One Condition
1836 Peroxiredoxin-6 PRDX6 Q-TOF 197 11 P30041 1.80 0.00001
1826 Heat shock protein beta-1 HSPB1 Q-TOF 324 11 P04792 1.74 0.00031
1506 26S proteasome non-ATPase regulatory subunit 14 PSMD14 Q-TOF 139 6 O00487 1.73 0.02200
1745 Cathepsin D CTSD Q-TOF 195 8 P07339 1.68 0.00002
1422 Reticulocalbin-1 RCN1 Q-TOF 432 19 Q15293 1.67 0.00027
1422 Vimentin VIM Q-TOF 68 3 P08670 1.67 0.00027
1643 Electron transfer flavoprotein subunit alpha, mitochondrial ETFA Orbitrap 1130 47 P13804 1.62 0.00035
1181 Eukaryotic initiation factor 4A-I EIF4A1 Q-TOF 138 5 P60842 −1.53 0.00300
748 Pyruvate kinase isozymes M1/M2 PKM2 Q-TOF 236 7 P14618 −1.59 0.00074
1304 Spermine synthase SMS Q-TOF 116 5 P52788 −1.62 0.00049
1304 Vimentin VIM Q-TOF 83 5 P08670 −1.62 0.00049
1402 Transaldolase TALDO1 Q-TOF 279 7 P37837 −1.66 0.01100
1274 Actin, cytoplasmic 2 ACTG1 Q-TOF 296 9 P63261 −1.76 0.00100
1395 Annexin A2 ANXA2 Q-TOF 122 4 P07355 2.16 0.00300 2.13 0.00930
2243 Actin-related protein 2/3 complex subunit 5 ARPC5 Q-TOF 175 5 O15511 1.91 0.00230 1.57 0.02100
1653 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial ECH1 Orbitrap 820 21 O15144 1.53 0.00001
1278 Protein SET SET Q-TOF 116 3 Q01105 −1.54 0.00620
980 Glucose-6-phosphate 1-dehydrogenase G6PD Q-TOF 279 14 P11413 −1.57 0.00000
1302 Sialic acid synthase NANS Q-TOF 208 6 Q9NR45 −1.60 0.00000
783 78 kDa glucose-regulated protein HSPA5 Orbitrap 887 31 P11021 3.00 0.00100
2045 Desmoplakin DSP Q-TOF 504 18 P15924 2.87 0.00460
1124 Actin, cytoplasmic 2 ACTG1 Q-TOF 383 14 P63261 1.75 0.00380
1124 POTE ankyrin domain family member E POTEE Q-TOF 269 7 Q6S8J3 1.75 0.00380
2363 Actin-related protein 2/3 complex subunit 5-like protein ARPC5L Orbitrap 961 36 Q4R5P2 1.73 0.00860
2363 Heat shock protein beta-6 HSPB6 Orbitrap 554 28 O14558 1.73 0.00860
837 Catalase CAT Q-TOF 176 9 P04040 1.68 0.00940
837 Pyruvate kinase isozymes M1/M2 PKM2 Q-TOF 151 6 P14618 1.68 0.00940
1174 Fumarate hydratase, mitochondrial FH Q-TOF 244 5 P07954 1.64 0.03400
1783 Endoplasmic reticulum resident protein 29 ERP29 Q-TOF 238 7 P30040 1.55 0.05000
1864 Ubiquitin carboxyl-terminal hydrolase isozyme L1 UCHL1 Q-TOF 121 6 P09936 −1.50 0.02900
1786 Phosphoglycerate mutase 1 PGAM1 Q-TOF 65 3 P18669 −1.53 0.00009
1779 Endoplasmic reticulum resident protein 29 ERP29 Orbitrap 447 14 P30040 −1.55 0.01100
1779 Beta-hexosaminidase subunit beta HEXB Orbitrap 533 21 P07686 −1.55 0.01100
1779 Nicotinamide N-methyltransferase NNMT Orbitrap 415 19 P40261 −1.55 0.01100
1005 Tubulin beta chain TUBB Q-TOF 311 10 P07437 −1.56 0.00035
1416 Annexin A1 ANXA1 Q-TOF 343 13 P04083 −1.56 0.00500
1728 Cathepsin D CTSD Q-TOF 175 8 P07339 −1.56 0.00900
1261 Fructose-bisphosphate aldolase A ALDOA Q-TOF 196 8 P04075 −1.57 0.00380
1437 Glyceraldehyde-3-phosphate dehydrogenase GAPDH Q-TOF 222 7 P04406 −1.61 0.01100
1225 Isocitrate dehydrogenase [NADP] cytoplasmic IDH1 Q-TOF 375 14 O75874 −1.63 0.02300
1026 Aldehyde dehydrogenase, mitochondrial ALDH2 Q-TOF 185 5 P05091 −1.65 0.00004
1026 Xaa-Pro dipeptidase PEPD Q-TOF 184 5 P12955 −1.65 0.00004
1026 Tubulin beta-4B chain TUBB4B Q-TOF 140 6 P68371 −1.65 0.00004
1985 Peroxiredoxin-2 PRDX2 Q-TOF 201 10 P32119 −1.65 0.00380
970 Protein disulfide-isomerase A3 PDIA3 Q-TOF 409 19 P30101 −1.70 0.00200
970 Vimentin VIM Q-TOF 158 4 P08670 −1.70 0.00200
1618 Annexin A5 ANXA5 Q-TOF 175 11 P08758 −1.71 0.00002
1182 Desmin DES Q-TOF 415 18 P17661 −1.77 0.01400
1182 Vimentin VIM Q-TOF 415 18 P08670 −1.77 0.01400
1441 Glyceraldehyde-3-phosphate dehydrogenase GAPDH Q-TOF 246 6 P04406 −1.77 0.00650
1942 Glutathione S-transferase P GSTP1 Q-TOF 329 10 P09211 −1.82 0.00094
1938 Glutathione S-transferase P GSTP1 Orbitrap 1357 40 P09211 −1.85 0.00000
992 Vimentin VIM Orbitrap 2287 97 P08670 −1.87 0.00400
1368 Actin, cytoplasmic 1 ACTB Q-TOF 215 8 P60709 −2.02 0.00056
1001 Tubulin beta chain TUBB Q-TOF 213 6 P07437 −2.03 0.00010
1151 Protein disulfide-isomerase P4HB Q-TOF 121 3 P07237 −2.04 0.00270
1151 Ribonuclease inhibitor RNH1 Q-TOF 86 3 P13489 −2.04 0.00270
1151 Vimentin VIM Q-TOF 40 2 P08670 −2.04 0.00270
1556 l-lactate dehydrogenase B LDHB Orbitrap 234 7 P07195 −2.05 0.00310
1556 Inorganic pyrophosphatase PPA1 Orbitrap 376 21 Q15181 −2.05 0.00310
1556 Tubulin beta-6 chain TUBB6 Orbitrap 239 8 Q9BUF5 −2.05 0.00310
1436 Glyceraldehyde-3-phosphate dehydrogenase GAPDH Q-TOF 206 6 P04406 −2.07 0.00900
1608 Annexin A4 ANXA4 Q-TOF 481 28 P09525 −2.17 0.00011
1485 Annexin A1 ANXA1 Q-TOF 108 4 P04083 −2.26 0.00032
1485 Malate dehydrogenase, cytoplasmic MDH1 Q-TOF 99 3 P40925 −2.26 0.00032

Nb: number; Sup/Dpl: Supplemented vs. Depleted.