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. 2018 Feb 5;215(2):459–479. doi: 10.1084/jem.20171739

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© 2018 Ben Youssef et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 1. — Vα7.2⁺ CD161^high T cell frequencies in neonates. (A) Quantification of the Vα7.2-Jα33 rearrangement (TRAV1-2 TRAJ33) by qPCR. Vα7.2-Jα33 expression was normalized to Cα expression in FACS-sorted Vα7.2⁺ CD161^high and Vα7.2⁺ CD161⁻ cells from three cord blood and two adult blood samples. The median from adult blood Vα7.2⁺ CD161^high populations was arbitrarily set to 100%. (B) Quantification of the canonical MAIT TCRα reads (TRAV1-2 TRAJ33/20/12 and 12–amino acid–long CDR3; left) and GEM TCRα reads (TRAV1-2 TRAJ9 and 13–amino acid–long CDR3; right) in sorted Vα7.2⁺ CD161^high and Vα7.2⁺ CD161⁻ populations from three cord blood and three adult blood samples. (C) Representative Vα7.2⁺ CD161^high cell staining in a neonate and a healthy adult control. Numbers indicate the percentage of Vα7.2⁺ and Vα7.2⁻ CD161^high cells among the CD3⁺ CD4⁻ γδ⁻ T cell gate. (D) Statistical dot plots showing the percentage of Vα7.2⁺ CD161^high cells among CD3⁺ T lymphocytes in 153 neonates and 36 healthy adult controls. Geometric means (horizontal bars) and statistical significance (Mann–Whitney test) are indicated. (E) Individual values and geometric means (horizontal bars) of frequencies of Vα7.2⁺ CD161^high, Vα7.2⁻ CD161^high, NKT, and conventional CD4 and CD8 T cells in each group of neonates (GA 24–27 wk, n = 28; GA 28–31 wk, n = 48; GA 32–36 wk, n = 41; >37 wk, n = 36). Differences between means were analyzed by one-way ANOVA with posttest for linear trend. (F) Correlation between the frequencies of Vα7.2⁺ CD161^high cells in 115 neonates and their mothers at birth. (G) Correlation between the frequencies of Vα7.2⁺ CD161^high, Vα7.2⁻ CD161^high, and NKT cells in 153 neonates at birth. The percentage of cells was transformed by using log to base 10 to perform statistical analysis. Spearman rank correlation coefficients (r) are indicated. (H) Correlation of the frequencies of Vα7.2⁺ CD161^high cells (left), Vα7.2⁻ CD161^high cells (medium), and NKT cells (right) between 21 twin pairs at birth (4 monozygotic and 17 dizygotic pairs, indicated by asterisks and circles, respectively). *, P < 0.01; **, P < 0.001; ***, P < 0.0001.