Fig. 3.

Effects of Sox5 modulation on dmrt1bY gene expression. Analysis of dmrtbY a transcriptional regulation after sox5 transient transfection in either spermatogonial or fibroblast medaka cell lines at 24 or 48 h post-transfection. Dataset results of four independent transfections. Statistical significance was assessed with the Wilcoxon–Mann–Whitney test (N = 4; * p value ≤ 0.05, ** p value ≤ 0.01). b Overexpression of sox5 was stimulated in fish eggs. The expression of dmrt1bY and germ-cell markers (nanos2, nanos3, dead-end, vasa, tra2a, and piwi) were monitored at stage 18 of development and compared to wild-type fish embryos. Dataset results of three independent transfections. Statistical significance was assessed with the t-test (N = 3 and each replicate is a pool of 25 eggs; * p value ≤ 0.05, ** p value ≤ 0.01). ns non-significant, OLF Oryzias latipes fibroblast, Sg3 Oryzias latipes spermatogonial cell