Fig. 3. (A) Determination of the dissociation constant of the Mg2+-4 adduct by measuring the fluorescence intensity of compound 4 (10 μM, H2O : EtOH 1 : 1) at increasing concentrations of Mg2+. Values represented as means ± s.e.m. (n = 3). Kd: 92 ± 30 μM. (B) Emission spectra of compound 4 (50 μM) upon incubation with 2 mM free Mg2+ (green) and 2 mM Mg-ATP (grey); λexc.: 450 nm. (Inset) Quantification of the fluorescence intensities for both conditions. Values represented as means ± s.d. (n = 3).
