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. 2018 Jan 25;9:2. doi: 10.3389/fpls.2018.00002

FIGURE 1.

FIGURE 1

Reverse transcription PCR and western blot analysis of phloem exudates from Arabidopsis. (A) Total RNA was collected from phloem exudates and rosette leaves of Col-0 to detect AtACBP3 and controls (UBC9, rbcL, and rbcS) transcripts. Original images of the analysis are available in Supplementary Figure S2. (B) Western blot analysis using anti-AtACBP3 antibodies on 30 μg total phloem exudate proteins from the acbp3 mutant, wild-type Col-0 and acbp3 scion grafted on AtACBP3-OE rootstock (acbp3/AtACBP3-OE) (left). A Coomassie Blue-stained gel loaded with the same amount of protein in western blot analysis is shown (right). (C) Western blot analysis using anti-AtACBP3 antibodies on 30 μg total root proteins collected from AtACBP3-OE/acbp3, Col-0/acbp3, acbp3 (left). A Coomassie Blue-stained gel loaded with the same amount of protein in western blot analysis is shown below (right). Arrowheads indicate the 39-kDa cross-reacting AtACBP3 band. These experiments were repeated twice with consistent results.