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. 2018 Jan 25;9:2. doi: 10.3389/fpls.2018.00002

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Copyright © 2018 Hu, Lung, Ye and Chye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding isotherms of recombinant (His)₆-AtACBP3 titrated with C18:2- and C18:3-CoA esters at 25°C in isothermal titration calorimetry. The panel shows raw data of 30 μM recombinant (His)₆-AtACBP3 titrated with 600 μM of C18:2-CoA ester, pH 7.0 (A); C18:2-CoA ester, pH 6.4 (B); C18:3-CoA ester, pH 7.0 (C) and C18:3-CoA ester, pH 6.4 (D). Each assay had three technical repeats and was repeated at least twice, each using independently prepared (His)₆-AtACBP3.