Table 1.
Dissociation constants between (His)6-AtACBP3 and acyl-CoA esters.
| Acyl-CoA esters | pH | KD (μM) | n (kcal/mol) |
|---|---|---|---|
| C12:0 | 7.0 | 33.1 ± 13.0 | 1.0 ± 0.3 |
| C12:0 | 6.4 | 6.1 ± 0.9 | 1.0 ± 0.0 |
| C14:0 | 7.0 | 4.0 ± 0.6 | 1.5 ± 0.0 |
| C14:0 | 6.4 | 3.3 ± 0.8 | 1.2 ± 0.0 |
| C16:1 | 7.0 | 5.1 ± 1.9 | 1.2 ± 0.1 |
| C16:1 | 6.4 | 9.7 ± 2.1 | 1.63 ± 0.0 |
| C17:0 | 7.0 | 21 ± 4.7 | 1.8 ± 0.1 |
| C17:0 | 6.4 | 3.7 ± 1.1 | 2 ± 0.0 |
| C18:2 | 7.0 | 13.4 ± 1.4 | 0.5 ± 0.0 |
| C18:2 | 6.4 | 6.4 ± 1.0 | 0.9 ± 0.0 |
| C18:3 | 7.0 | 8.6 ± 2 | 0.6 ± 0.0 |
| C18:3 | 6.4 | 9.0 ± 0.1 | 0.7 ± 0.0 |
Each assay had three technical repeats and was repeated at least twice using (His)6-AtACBP3 that was independently prepared. Standard deviations were calculated based on readings of three technical repeats.