Table 1.
Primer sequences used for qRT-PCR amplification
| Gene | Forward primer | Reverse primer | Accession number | Product size(bp) |
|---|---|---|---|---|
| β-actin | TGCTGTCCCTGTATGCCTCT | TTTGATGTCACGCACGATTT | NM_031144.3 | 224 |
| IL-1β | ACCTGTGTCTTTCCCGTGG | TCATCTCGGAGCCTGTAGTG | NM_031512.2 | 159 |
| TNFα | GCCTCCCTCTCATCAGTTCTA | GGCAGCCTTGTCCCTTG | NM_012675.3 | 246 |
| IL-6 | AGTTGTGCAATGGCAATTCTGA | AGGACTCTGGCTTTGTCTTTCT | NM_012589.2 | 223 |
| iNOS | TTCCAGAATCCCTGGACAAG | TGGTCAAACTCTTGGGGTTC | NM_012611.3 | 180 |
| COX-2 | AGAAGGAAATGGCTGCAGAA | GCTCGGCTTCCAGTATTGAG | NM_017232.3 | 194 |