Table 1.
Real‐time PCR array analysis of T‐cell and B‐cell genes in lung homogenate of mice exposed to 85% O2 for 12 days from P3 to P15
| Gene Symbol | Gene Description | Fold Change of 85% O2 – 2‐week group (compared with 21% O2 – 2 weeks) |
|---|---|---|
| Genes involved in B‐cell activation | ||
| Cr2 | Complement receptor 2 | −4.73 |
| Igbp1b | Immunoglobulin (CD79A) binding protein 1b | −12.46 |
| B‐cell differentiation | ||
| Il10 | Interleukin 10 | −6.06 |
| Il4 | Interleukin 4 | −5.31* |
| Nkx2‐3 | NK2 transcription factor related, locus 3 (Drosophila) | −5.32 |
| Rag1 | Recombination activating gene 1 | −4.16* |
| Regulators of T‐cell activation | ||
| Cd1d1 | CD1d1 antigen | −13.05* |
| Cd3d | CD3 antigen, delta polypeptide | −4.20 |
| Cd8a | CD8 antigen, alpha chain | −5.43 |
| Irf4 | Interferon regulatory factor 4 | −4.86 |
| Prlr | Prolactin receptor | −22.70 |
| T‐cell proliferation | ||
| Cd3e | CD3 antigen, epsilon polypeptide | −5.51 |
| T‐cell differentiation | ||
| Ap3b1 | Adaptor‐related protein complex 3, beta 1 subunit | −12.68 |
| Cd4 | Cd4 antigen | −39.71 |
| Il27 | Interleukin 27 | −6.75 |
| Th1/Th2 differentiation | ||
| Cd28 | CD28 antigen | −7.06* |
| Cd40lg | CD40 ligand | −17.30 |
| Other genes related to immune cell activation | ||
| H60a | Histocompatibility 60a | −14.90 |
| Overexpressed gene | ||
| Cdkn1a | Cyclin‐dependent kinase inhibitor 1A (P21) | 7.70* |
Genes in the 85% O2 2‐week group were expressed as the fold change of expression of the same gene in the RA 2‐week group (control group; gene expression in the control group = 1.0). Genes that are under (≤4.0) or over (≥4.0) expressed (relative to RA 2‐week group or the control group) or significant are shown (n = six in each group); *P < 0.05 versus RA 2 weeks by Student's t‐test. A negative sign prefixes underexpressed genes. Housekeeping genes – Gapdh and Hprt; some genes act at more than one phase of B‐/T‐cell activation.