Table 2.
Real‐time PCR array analysis of T‐cell and B‐cell genes in lung homogenate of mice exposed to 85% O2 for 12 days from P3 to P15
| Gene symbol | Gene description | Fold change of 85% O2 – 3‐month group (compared with 85% O2 – 2 weeks) |
|---|---|---|
| Overexpressed genes at 3 months | ||
| Clcf1 | Cardiotrophin‐like cytokine factor 1 | 4.78 |
| Cd1d1 | CD1d1 antigen | 4.24 |
| Cd3d | CD3 antigen, delta polypeptide | 4.27* |
| Sit1 | Suppression inducing transmembrane adaptor 1 | 4.12 |
| Cd3e | CD3 antigen, epsilon polypeptide | 7.96* |
| Ap3b1 | Adaptor‐related protein complex 3, beta 1 subunit | 5.45 |
| Cd4 | Cd4 antigen | 6.59 |
| Il27 | Interleukin 27 | 4.67* |
| Cd28 | CD28 antigen | 5.15* |
| Cd40lg | CD40 ligand | 5.37* |
| Tnfsf13b | Tumor necrosis factor (ligand) superfamily‐13b | 5.10* |
| Ifnγ | Interferon gamma | 7.10* |
| Underexpressed genes at 3 months | ||
| Cdkn1a | Cyclin‐dependent kinase inhibitor 1A (P21) | −4.29* |
| Rag1 | Recombination activating gene 1 | −4.81 |
Genes in the 85% O2 3‐month group were expressed as a fold change of expression of the same gene in the 85% O2 2‐week group (control group; gene expression in the control group = 1.0). Genes that are under (≤4.0) or over (≥4.0) expressed (relative to 85% O2 2 weeks or the control group) or significant are shown (n = six in each group); *P < 0.05 versus RA–2 weeks by Student's t‐test. A negative sign prefixes underexpressed genes. Housekeeping genes – Gapdh and Hprt.