Table 3. mVIM and mCCNA1 Detection in FFPE biopsies of upper GI tract pathologies.
mVIM and mCCNA1 were assayed in microdissected FFPE biopsies that captured each of the histologies shown. BE (Barrett’s esophagus); IM (intestinal metaplasia); GEJ (gastroesophageal junction). Samples were scored as methylated for mVIM >1.05%, mCCNA1>3.12% (the cutpoints established in ROC analysis of esophageal brushings assayed for each marker). Samples were positive for the panel of mCCNA1 plus mVIM if either marker tested positive. Entries indicate percent positive samples (%) and total number of individuals tested (n).
| mCCNA1 | mVIM | Either mVIM or mCCNA1 |
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|---|---|---|---|---|---|---|
| % | n | % | n | % | n | |
| BE (IM) | 75% | 20 | 90% | 21 | 90% | 21 |
| GEJ/cardia with IM (<1cm extent) | 67% | 9 | 70% | 10 | 80% | 10 |
| Non-IM columnar metaplasia concurrent with BE | 11% | 9 | 30% | 10 | 30% | 10 |
| GEJ/cardia without IM (including 15 cases with chronic carditis) | 0% | 53 | 0% | 54 | 0% | 55 |
| Distal normal squamous esophagus from control patients without glandular metaplasia | 0% | 24 | 0% | 24 | 0% | 24 |
| Eosinophilic esophagitis | 0% | 12 | 0% | 15 | 0% | 15 |
| Gastric fundic mucosa without IM | 4% | 24 | 0% | 24 | 4% | 24 |
| Intestinal metaplasia of stomach | 14% | 7 | 11% | 9 | 22% | 9 |
| Helicobacter pylori gastritis without IM | 15% | 13 | 8% | 13 | 15% | 13 |