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. Author manuscript; available in PMC: 2018 Jan 30.
Published in final edited form as: Virology. 2013 May 29;443(1):11–19. doi: 10.1016/j.virol.2013.04.033

Figure 5.

Figure 5

Mutations of E7 in late promoter activation. a. A diagram of important domains in the E7 protein, with the locations of mutations used in this study. b. HPV16-containing keratinocytes were cotransfected with luciferase reporters for the late promoter and expression vectors for E7 mutants. Following culture in monolayer or MC for 24 hours, luciferase activities were measured and normalized as described in Figure 1. V, pcDNA empty vector; CVQ, CVQ68-70AAA. Values represent the means of 9-14 independent experiments and bars represent ± one standard error of the mean. Samples marked with (*) were significantly different from wild-type E7 in the corresponding culture condition (monolayer or MC, p<0.05). c. To determine expression levels of mutant E7s, U2OS cells were transfected with the indicated expression vectors for E7 and subjected to Western blotting analysis.