Figure 8.
Promoter inhibition by E2F1 is cell cycle dependent. HPV16-containing keratinocytes were cotransfected with luciferase reporters for the late promoter and expression vectors for E7 or the indicated E2F expression vectors. Following culture in monolayer or MC for 24 hours, luciferase activities were measured and normalized as described in Figure 1. V, pcDNA empty vector; pGL, empty luciferase reporter vector. Values represent the means of 4-8 independent experiments and bars represent ± one standard error of the mean. *, p<0.01; n.s., not significant.