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. Author manuscript; available in PMC: 2018 Jan 30.
Published in final edited form as: Science. 2012 Sep 13;338(6109):975–980. doi: 10.1126/science.1228309

Fig. 4.

Fig. 4

Specificity of AP-1–IRF complexes that cooperatively assemble on AICE motifs. (A to D) EMSAs using the Bcl11b probe and nuclear extracts from 293T cells overexpressing the indicated proteins: (A) c-Jun, BATF, IRF4; (B) FosL2, JunB, IRF4; (C) JunB, BATF, IRF8; or (D) JunB, BATF, IRF3, and IRF4. Complexes were supershifted with indicated antibodies. Red and blue arrows mark the ternary and quaternary protein-DNA complexes, respectively, as in Fig. 1. Data are representative of at least two independent experiments. (E to G) ChIPseq analysis of in vitro differentiated TH0 and TH2 cells (42 hours) and LPS-activated dendritic cells (6 hours) with antibodies directed against IRF4. Highly represented motifs and their respective E values within the IRF4 cistrome in TH0 (241 peaks), in TH2 (725 peaks), and in dendritic cells (10,364 peaks) are displayed.