Fig. 1.

GT reaction schematic. (Top) A simplified reaction where a GT catalyzes the transfer of a carbohydrate moiety from a specific donor substrate to an acceptor molecule. For screening of sugar-nucleotide donors, a GT is incubated with a panel of donor sugars in the absence of an acceptor substrate (Bottom), and the UDP released upon background hydrolysis of the UDP-sugar is detected using the UDP-Glo™ Glycosyltransferase Assay, where UDP is enzymatically converted to Adenosine triphosphate (ATP), followed by a luciferase reaction which emits light. The luminescence detected by the luminometer is directly proportional to UDP concentration. This figure is available in black and white in print and in color at Glycobiology online.