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. 2018 Jan 30;8:1850. doi: 10.1038/s41598-018-19351-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spatiotemporal resolution of PLA interactions between importin beta-1 and the indicated partners during mitotic progression. PLA panels (100 × objective) depict the sites of interaction of importin beta-1 with partners validated in Fig. 3, relative to the mitotic apparatus (alpha-tubulin), chromosomes or reforming nuclei (DAPI): (a) RAN; (b) RANBP1; (c) RANBP2; (d) NUSAP; (e) BUB3. Representative stages for each combination are shown and details are given in the text. Bar, 5 μm.