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. 2018 Jan 30;8:1854. doi: 10.1038/s41598-018-19758-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in SMA⁺ perivascular cells and myofibroblasts in the aftermath of MI. (a) Example of 3D reconstructions of the microvasculature (red) merged with the segmentation of the SMA⁺ cells channel (grey) in a basal and an infarcted 7 days post MI case. (b) Changes in SMA⁺ related metrics, i.e. the percentage of vessels with a SMA⁺ coat along with the corresponding thickness of the coat. (c) Analysis of the number of SMA⁺ cells of which the SMA⁺ coat covering the microvessels consists. (d) Analysis of the cardiac tissue by means of indicators of tissue fibrosis, i.e. the Damage Index (percentage of SMA⁺ volume that are not associated with vessels), and number of myofibroblasts per mm³ of tissue.