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. 2018 Jan 30;9:428. doi: 10.1038/s41467-017-02650-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Expression profile of tbx5a-positive cardiomyocytes in embryonic zebrafish hearts. a–f Whole-mount immunofluorescence of tbx5a:GFP;myl7:mbmCherry double transgenic zebrafish hearts at 32 a–c (n = 5/5) and 56 hours postfertilisation (hpf) d–f (n = 3/3). g–l Confocal optical sections of tbx5a:GFP;myl7:mbmCherry hearts at 72 hpf g–i (n = 5/5), 4 days postfertilisation (dpf) j–l (n = 9/9), and 5 dpf (m–o; n = 6/6). GFP (green) labels tbx5a⁺ cells and mCherry (red) marks cells expressing the pan-myocardial marker myosin light chain 7 (myl7). Shown are ventral views, cranial is to the top. At 32 hpf all cardiomyocytes are tbx5a:GFP⁺ but at 56, 72 hpf, 4, and 5 dpf tbx5a:GFP⁻ cardiomyocytes can be observed in the distal ventricle (arrowheads). The atrioventricular canal and large portions of the atrium are also GFP⁺. at, atrium; v, ventricle; Scale bars, 10 μm