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. 2018 Jan 30;9:428. doi: 10.1038/s41467-017-02650-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Expression profile of tbx5a-positive cardiomyocytes in adult zebrafish hearts. a, b Sagittal sections through tbx5a:GFP adult uninjured heart immunostained with GFP (green) and Myosin Heavy Chain (MHC; red). Nuclei are counterstained with DAPI (blue). c–e Zoomed views of boxed area in a. b, d, f Single channels for GFP. The trabecular myocardium is tbx5a:GFP⁺, whereas the cortical layer is tbx5a:GFP⁻. Note tbx5a:GFP⁻ cardiomyocytes (arrowheads) in the basal part of the ventricle close to the atrioventricular canal (n = 13/13). at, atrium; cort, cortical layer; trab, trabecular layer. Scale bars, a, b 100 μm and c–f 25 μm