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. 2018 Jan 30;9:428. doi: 10.1038/s41467-017-02650-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Genetic ablation of tbx5a-derived ventricular cardiomyocytes. a tbx5a⁺ ventricular cardiomyocytes were genetically ablated in tbx5a:CreER^T2;vmhcl:loxP-tagBFP-loxP-mCherry-NTR double transgenic zebrafish. Recombination was induced by administration of 4-Hydroxytamoxifen (4-OHT). Cell ablation was induced by administration of Metronidazole (Mtz) from 4 to 7 days postfertilisation. b, c Ventral views of larval hearts at 4 dpf (maximal projection and optical section, respectively). Anterior is to the top. The proximal ventricle, including primordial layer and trabeculae, is completely mCherry⁺ and the distal ventricle is blue (tagBFP⁺); n = 7/7. d, e Sagittal section of the ventricle of an adult recombined heart. Most cells are mCherry⁺. Only the tbx5a⁻ region is tagBFP. n = 7/7. f, g, Sagittal section of a Mtz-treated fish. Most of the cardiomyocytes are tagBFP⁺; n = 6/6. h, Quantification of the percentage of myocardium that is tagBFP⁺ (SHF-derived), mean±SD; *** P < 0.0001 by two-tailed unpaired t-test. at, atrium; prim, primordial layer; SHF, second heart field; trab, trabeculae; v, ventricle. Scale bars, 25 μm