Fig. 3.

Neutralization of TBEV by antibody 19/1786. Dose-dependent neutralization curves of TBEV treated with IgG 19/1786 (a) and Fab 19/1786 (b). Error bars represent standard deviation of the measurements (IgG n = 5; Fab n = 2). EC₅₀ values with standard error of the mean are shown in the graph. c Dose-dependent neutralization curves of TBEV and its mutants treated with IgG 19/1786. Mutations in domain I (red circle—double mutant; blue triangle—quadruple mutant) and domain II (magenta triangle—double mutant; green diamond—quadruple mutant) affect the neutralization activity of Fab 19/1786 compared to the wild-type virus (black square). The mutations in the domains are listed in the top left corner. The error bars represent the standard deviation of the measurements (n = 3). Calculated EC₅₀ values (µg ml⁻¹) with standard errors are listed in the bottom right corner