Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 30;8:1838. doi: 10.1038/s41598-018-20216-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Immunoprecipitation with anti-P2X₁ antibody. Antibodies to P2X₁ were immobilized onto resin beads and then incubated with mouse bladder lysates to IP the antigen and co-IP interacting proteins. Proteins that were bound (IP: 2.5 µg protein/lane) or did not bind (FT: 25 µg protein/lane) to the beads were resolved by SDS-PAGE, and Western blots were probed with A) P2X_1, B) P2X₄ or C) Nt5e antibodies. (A) Left and right panels show P2X₁ immunoblots on IP and FT lysates from wild type and P2X₄−/− mice. Monomeric P2X₁ can be seen highly concentrated in the pulldown fraction at 50 kDa. Little P2X₁ appears in FT. (B) P2X₄ antibody detects P2X₄ as a band at 70 kDa in wild type, but is completely absent in P2X₄−/− mice. The antibody shows minor cross-reactivity to possibly P2X₁. Note, there is no evidence of the 70 kDa band in the IP lane. (C) An antibody to 5′-nucleotidase (Nt5e) demonstrates that pulldown with anti-P2X₁ is ‘clean’ with no non-specific protein binding evident in the IP lanes.