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. 2018 Jan 30;8:1838. doi: 10.1038/s41598-018-20216-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myography indicates that BSM from P2X₄−/− mice have normal contractility in response to electrical field stimulated neurotransmitter release. (A) Frequency train data on wild type bladder strips before and after atropine treatment; (B) Frequency train data on P2X₄−/− bladder strips before and after atropine treatment; (C) and (D) Quantitation of force from wild type (n = 12) and P2X₄−/− (n = 12) muscle strips. Data shown is mean ± SD.