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. 2018 Jan 30;8:1882. doi: 10.1038/s41598-018-20185-8

Figure 2.

Figure 2

Anti-C1q antibody ELISA. (A) A129 mice were infected with ZIKV strain FSS13025 and sacrificed at indicated days. Anti-C1q antibody was measured by ELISA. The cut-off value was 190.0 U/mL (dash line). (B) Serum samples were collected from CD-1 mice infected with ZIKV strain FSS13025 and measured for anti-C1q antibody. The cut-off values were 268.4 U/mL (dash line) for 15 dpi and 205.8 U/mL for 30 dpi. (C) Serum samples were collected at 0 dpi (Pre-infection) and 28 dpi from A129 mice infected with ZIKV strain FSS13025. The dash lines indicate the cut-off value 252.1 U/mL. (D) Serum samples were collected at 0 dpi (Pre-infection) and 11 dpi from cynomolgus macaques (Macaca fascicularis) infected with ZIKV strain H/PF/2013. The dash lines indicate the cut-off value 0.272. The data is shown by ELISA OD values since there was no calibration samples in the ELISA kit. (E) Serum samples were collected from BALB/c mice infected with WNV TX AR12–9793 at 21 dpi and measured for anti-C1q antibody. The cut-off values were 178.2 U/mL (dash line). Each dot shows the average values of duplicate wells. The lines between dots indicate that the samples were obtained form same animals. P values were calculated using Mann-Whitney U test (compared with negative control or pre-infection). Each cut-off value was determined by the mean plus 3 SDs of the negative controls (un-infected) or pre-infection.