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. 2018 Jan 30;8:1903. doi: 10.1038/s41598-018-20347-8

Table 1.

Physicochemical properties, microbial abundances and potential bacterial respiration determined in soil samples of the studied layers A14 (upper), A16 (intermediate) and A18 (lower). For each variable, mean values followed by different letters are significantly different (p ≤ 0.05).

A14 A16 A18
Soil physicochemical properties
pH 6.5–7.0a 6.5–7.0a 6.5–7.0a
SOM (%)1 3.03b 2.19a 2.43ab
TOC (%)2 1.76b 1.27a 1.41ab
N (%) 0.07a 0.08a 0.09a
TOC/N 24.04b 15.41a 15.12a
PLFA-based microbial abundance
Total abundance (μg PLFA g−1 dry soil) 515.72a 387.60a 411.25a
Bacteria (B) (μg PLFA g−1 dry soil) 289.22a 180.24a 130.95a
Actinobacteria (μg PLFA g−1 dry soil) 59.09a 36.49a 20.59a
Fungi (F) (μg PLFA g−1 dry soil) 18.93a 26.09a 26.75a
F/B 0.07a 0.15ab 0.20b
qPCR-based microbial abundance
Bacteria (B) (16S rRNA gene copy number g−1 dry soil) 1.45 × 107a 1.52 × 107a 1.62 × 107a
Archaea (A) (16S rRNA gene copy number g−1 dry soil) 8.70 × 105a 8.85 × 105a 1.32 × 106b
Fungi (F) (18S rRNA gene copy number g−1 dry soil) 2.25 × 104a 3.57 × 104a 8.25 × 104a
A/B 0.83a 0.83a 0.85a
F/B 0.59a 0.63a 0.62a
Potential bacterial respiration
gtlA (gene copy number g−1 dry soil) 7.73 × 103b 4.57 × 103a 3.76 × 103a

1SOM, soil organic matter.

2TOC, total organic carbon.