Fig. 1.

Loss of Gimap5 impairs CD4⁺ T cell survival and iTreg cell induction. Ten-week-old control Gimap5^WT/WT;Rag2^−/−;OT-II and Gimap5^sph/sph;Rag2^−/−;OT-II mice received either normal water or water containing 1 mg/mL ovalbumin ad libitum during a 5-week period. At 15 weeks of age, a the number of splenic CD4⁺ T cells and b the percentage of naïve (CD44^lo;CD62^hi) and memory-like (CD44^hi;CD62^lo) CD4⁺ T cells was determined using flow cytometry. c Frequency of iTregs (CD4⁺CD25⁺) in the mesenteric lymph nodes (MLN) of vehicle- and OVA-treated mice (n = 4). d Survival of peripheral (splenic) CD4⁺ T cells ex vivo in the presence of IL-7 (5 ng/mL) as determined by live/dead staining and flow cytometry (n = 3). Data represent mean values + SD of samples from individual mice; statistical significance is determined by Student’s two-tailed test