Fig. 4.

Loss of Gimap5 causes increased DNA damage in activated CD4⁺ T cells. a γH2AX expression in live WT or Gimap5^sph/sph CD4⁺ T cells following αCD3/αCD28 stimulation. b Survival as determined by viability stain of WT or Gimap5^sph/sph CD4⁺ T cells after αCD3/αCD28 stimulation (n = 4). Effect of lithium on γH2AX expression in WT or Gimap5^sph/sph CD4⁺ T cells, after 3 days stimulation with αCD3/αCD28. Plots depict live cells (c, d), while bar graphs (d) represent mean values ± SD (n = 6). All experiments were performed at least three times. Statistical significance is determined by Student’s two-tailed test