Table 1.
Genotypea | °C | nb | Rhythmic (%) | Periodc ± SEM | Powerd ± SEM |
---|---|---|---|---|---|
TUG x w1118 | 18 | 30 | 100 | 24.4 ± 0.2 | 222.2 ± 20.6 |
V38862 x w1118 | 18 | 28 | 93.8 | 24.2 ± 0.3 | 249.0 ± 19.8 |
V101740 x w1118 | 18 | 30 | 100 | 23.9 ± 0.1 | 268.3 ± 13.6 |
T37519 x w1118 | 18 | 27 | 100 | 23.8 ± 0.2 | 198.9 ± 14.7 |
TUG x V38862 | 18 | 23 | 85.4 | 23.9 ± 0.2 | 251.1 ± 24.8 |
TUG x V101740 | 18 | 24 | 91.2 | 25.6 ± 0.3 | 188.2 ± 15.2 |
TUG x T37519 | 18 | 19 | 91.7 | 24.6 ± 0.6 | 165.6 ± 21.4 |
TUG x w1118 | 25 | 31 | 100 | 24.0 ± 0.1 | 286.8 ± 12.5 |
V38862 x w1118 | 25 | 30 | 100 | 23.7 ± 0.04 | 314.5 ± 14.8 |
V101740 x w1118 | 25 | 32 | 100 | 23.8 ± 0.06 | 309.1 ± 15.3 |
T37519 x w1118 | 25 | 29 | 100 | 23.8 ± 0.09 | 256.8 ± 17.7 |
TUG x V38862 | 25 | 27 | 100 | 23.5 ± 0.07 | 293.5 ± 19.5 |
TUG x V101740 | 25 | 32 | 100 | 24.8 ± 0.2 | 268.7 ± 20.5 |
TUG x T37519 | 25 | 30 | 93.3 | 24.2 ± 0.1 | 219.0 ± 19.4 |
aFor each cross we set up two contemporaneous crosses using males from one genotype crossed to virgin females of the other genotype and vice-versa. All results are an average of crosses in both directions. Independent lines of RNAi-B52 were used (V38862, V101740, T37519). All flies were in the w1118 genetic background and the TUG flies co-expressed UAS-Dcr-2.
bNumber of flies that survived the entire testing period.
cFlies were entrained for 5 days in LD at the indicted temperature followed by 7 days in complete darkness (DD). Activity data collected during DD was used to calculate free-running periods.
dPower is a relative measure of rhythm strength.