Fig. 1.

α-Syn tetramers and related multimers in SH-SY5Y cells with GBA1 KO by the CRISPR/Cas9 system. (A) Cytosolic fractions from 1 mM DSG–cross-linked control and GBA1 KO cells were analyzed by Western blot using anti–α-Syn antibody to detect cross-linked α-syn. αS, α-synuclein. (B) Quantification of the cytosolic αS60 + 80 + 100:14 ratio in the GBA1 KO cells relative to the ratio in the control cells (n = 6). (C) Quantification of the ratio of DJ-1 dimer to monomer (n = 6). The error bars represent SEM. **P < 0.01; n.s., not significant.