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. 2018 Jan 8;115(4):798–803. doi: 10.1073/pnas.1700465115

Fig. 2.

Fig. 2.

Rescue effects of GSL reducing agent on GSL homeostasis and formation of α-syn tetramers and related multimers. (A) Fluorescent microscopic images of vehicle or 100 μM miglustat treatment for 3 d in control and GBA1 KO cells using anti-GBA1 and anti-GlcCer antibodies. (Scale bar, 10 μm.) (B) Quantification of GlcCer intensity divided by number of DAPI-stained cells relative to vehicle-treated control cells (n = 4). (C) Cytosolic fractions of DSG–cross-linked control and GBA1 KO cells with or without miglustat treatment. (D) Quantification of the α-syn multimers to monomer ratio for C (n = 6). (E) Quantification of the ratio of DJ-1 dimer to monomer (n = 6). The error bars represent SEM. *P < 0.05; **P < 0.01; n.s., not significant.