Fig. 4.

Otogl morphants are sensitive to infection with A. hydrophila. (A) Survival time course of MOC-injected and Otogl MO-injected tadpoles in 0.01× Marc’s Modified Ringer’s (MMR). (B) Survival time course of MOC-injected and Otogl MO-injected tadpoles in 0.01× MMR containing 1.5 × 10⁸ cfu/mL of A. hydrophila (at time point 0 h). Individual points represent mean survival levels from three independent experiments, and error bars represent the SEM. (C) Bar chart comparing the frequency of GFP-expressing A. hydrophila bacteria located within the tadpole in MOC- and Otogl MO-injected tadpoles fixed and sectioned at the 34-h time point. Bars represent mean number of bacteria found within MOC (n = 3 tadpoles)- and Otogl MO (n = 5 tadpoles)-injected tadpoles. Error bars represent SEM and P = 0.0179 (one-tailed Mann–Whitney U test). (D) Representative images of sections of MOC- and Otogl MO-injected tadpoles following exposure to GFP-expressing A. hydrophila bacteria at the 34-h time point. The white line on the Left image represents the apical surface membrane from brightfield images (Right). (Scale bar: 10 μm.)