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. 2018 Jan 8;115(4):E762–E771. doi: 10.1073/pnas.1715865115

Fig. 1.

Fig. 1.

Neural induction analysis in HD and CTR iPSCs following striatal differentiation. (A) Immunocytochemistry for OCT4 and PAX6 at DIV8 and 15 of differentiation in Q21n1 and three HD lines (Q60n5, Q109n1, Q180n1). (Scale bar, 100 μm; Inset, 50 μm.) (B) Counts of OCT4+ (red column) and PAX6+ (green column) cells by the Automatic Nuclei Counter plug (ITCN) ImageJ plugin both at DIV8 and 15. (C) Counts of OCT4+ (red column) cells at DIV 8 and 15 and PAX6+ (green column) cells at DIV 15 by ITCN in CTR and HD lines. (One-way ANOVA, OCT4: #P < 0.01 between HD and CTRs at DIV8, one-way ANOVA; OCT4 and PAX6: ***P < 0.001 between HD and CTRs at DIV15, one-way ANOVA; n = 3 biological experiments, data are represented as mean ± SEM.) (D) Graph of OCT4 and CAG length correlation for all seven HD lines/clones (Q60n5, Q60n8, Q109n1, Q109n4, Q109n5, Q180n1, and Q180n3) (r = 0.97, P = 6.4e-15 calculated using Pearson correlation).