Fig. 6.

EV production by adoptive transferred tumour-specific CD8⁺ T cells. a Cultured Thy-1.1⁺ DUC18 CD8 EV bound to latex beads were stained with the indicated mAbs, and subjected to flow cytometric analysis. b EV proteins from the supernatant of Thy-1.1⁺ DUC18 CD8⁺ T cells cultured with/without GW4869 (DMSO treatment as a control) were measured by BCA assay (**p < 0.01; error bars indicate SEM) (n = 3). The data were analysed by a two-tailed unpaired Student t-test. c Thy-1.1⁺ DUC18 CD8⁺ T cells cultured with/without GW4869 were transferred intravenously into CMS5a tumour-bearing BALB/c mice (n = 6 per group). The next day, resected CMS5a tumours were sectioned and stained with the indicated FITC-conjugated or PE-conjugated mAbs and DAPI (c), or PE-conjugated CD140a-, FITC-conjugated Thy-1.1- and APC-conjugated Sca-1-specific mAbs (d). The stained tumour sections were observed by fluorescent microscopy (c) or confocal laser scattering microscopy (d). Each photo is a representative image from 3–5 areas. The dotted circles and arrows indicate MSC areas (purple) and Thy-1.1⁺ EV-engulfed MSCs (white), respectively